Journal: International Journal of Oncology

Article Title: Synergistic and toxicity-reducing effects of acteoside as an adjuvant therapy of oxaliplatin against hepatocellular carcinoma

doi: 10.3892/ijo.2025.5751

Figure Lengend Snippet: Effects of OXA + ACT on protein expression in the HepG2 model mice. (A) Western blot analysis. Effects of ACT + OXA on (B) p-AKT1, (C) AKT1, (D) p-AKT1/AKT1, (E) p-PIK3R1, (F) PIK3R1, (G) p-PIK3R1/PIK3R1, (H) PRKCA, (I) PLCB4, (J) PTEN, (K) INPP4B and (L) INPP5D protein levels in HepG2 model mice. * P<0.05, ** P<0.01. ns, not significant; ACT, acteoside; OXA, oxaliplatin; p-, phosphorylated.

Article Snippet: The primary antibodies β-actin (cat. no. bs-0061R; 1:5,000), AKT1 (cat. no. bsm-52010R; 1:500), phosphorylated (p)-AKT1 (cat. no. bs-5194R; 1:500), PIK3R1 (cat. no. bs-0128R; 1:500), p-PIK3R1 (cat. no. bs-6417R; 1:500), PRKCA (cat. no. bsm-54393R; 1:500), INPP5D (cat. no. bs-3567R; 1:500), PTEN (cat. no. bsm-33319M; 1:500). and the secondary antibodies goat anti-rabbit IgG H&L (cat. no. bs-0295G; 1:5,000) and anti-mouse IgM, HRP conjugated (cat. no. bs-0368G-HRP; 1:5,000) purchased from Beijing Bioss Biotechnology Co., Ltd ( http://bioss.com.cn/ ).

Techniques: Expressing, Western Blot

Journal: Current Issues in Molecular Biology

Article Title: Evaluation of the Expression of IDO and PTEN in Human Kidney Cancer

doi: 10.3390/cimb47050359

Figure Lengend Snippet: qPCR analysis of mRNA for PTEN expression in renal cancer tissues. Mean value of the 20 analyzed normal and RCC tissue sample pairs. For each PCR measurement, 40 µg of cDNA was used, and GAPDH served as the housekeeping gene. Statistical significancy was observed between the healthy and tumorous samples using the two-way ANOVA test with Sidak multiple comparison test ( p = 0.0271 for PTEN). * shows significant difference between tumorous and normal tissue samples.

Article Snippet: Blotting membranes were blocked with 5% milk-TBS-Tween for 1 h at room temperature and then incubated overnight with the specific primary antibodies at 4 °C (IDO, D5J4E in 1:1000 dilution, BOSTER Biological Technology, Pleasanton, CA, USA; PTEN (D4.3) XP(R) Rabbit mAB, in 1: 1000 dilution, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Comparison

Journal: Current Issues in Molecular Biology

Article Title: Evaluation of the Expression of IDO and PTEN in Human Kidney Cancer

doi: 10.3390/cimb47050359

Figure Lengend Snippet: Relative mRNA expression of PTEN in human RCC tissue samples. The IDO mRNA level was normalized to the GAPDH housekeeping gene. The mRNA levels of the RCC sample were compared to the adjacent healthy tissue samples; then, the log 2 fold change was displayed. A total of 13 samples show upregulation (above the 0 line, red dots) of PTEN in the RCC tissue samples. Blue dots show the group of the downregulated samples for PTEN of the studied samples.

Article Snippet: Blotting membranes were blocked with 5% milk-TBS-Tween for 1 h at room temperature and then incubated overnight with the specific primary antibodies at 4 °C (IDO, D5J4E in 1:1000 dilution, BOSTER Biological Technology, Pleasanton, CA, USA; PTEN (D4.3) XP(R) Rabbit mAB, in 1: 1000 dilution, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing

Journal: Current Issues in Molecular Biology

Article Title: Evaluation of the Expression of IDO and PTEN in Human Kidney Cancer

doi: 10.3390/cimb47050359

Figure Lengend Snippet: Western blot analysis of the expression of protein for PTEN in CAKI-2 and A-498 cell lines. HPRT was used as a housekeeping protein. A total of 40 µg of proteins were loaded into polyacrylamide gel and separated by electrophoresis (SDS-PAGE); then, protein for PTEN was detected with a specific monoclonal antibody (PTEN (D4.3) XP(R) Rabbit mAB, Cell Signaling Technology, Danvers, MA, USA).

Article Snippet: Blotting membranes were blocked with 5% milk-TBS-Tween for 1 h at room temperature and then incubated overnight with the specific primary antibodies at 4 °C (IDO, D5J4E in 1:1000 dilution, BOSTER Biological Technology, Pleasanton, CA, USA; PTEN (D4.3) XP(R) Rabbit mAB, in 1: 1000 dilution, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Expressing, Electrophoresis, SDS Page

Journal: Current Issues in Molecular Biology

Article Title: Evaluation of the Expression of IDO and PTEN in Human Kidney Cancer

doi: 10.3390/cimb47050359

Figure Lengend Snippet: A representative figure of the expression of protein for IDO ( A ). and PTEN ( B ) in adjacent healthy (1N; 2N; 3N; 4N) and tumorous (1T; 2T; 3T; 4T) ccRCC tissue samples. The bands 1, 2, 3, and 4 representing the protein expression of IDO and PTEN are identical to patient numbers 10, 11, 5, and 3, respectively. A total of 40 µg of each protein sample isolated from tissue samples were loaded onto polyacrylamide gel and separated by electrophoresis (SDS-PAGE). A specific monoclonal antibody was used for PTEN (PTEN (D4.3) and IDO (IDO, D5J4E). The intensity of the protein bands was quantified using the Image Lab software (version 5.2.1, Bio-Rad Laboratories Inc., Hercules, CA, USA). ( A ): According to the band intensities, the first sample pair shows downregulated IDO expression, while in the other samples IDO was upregulated in the tumorous tissue sample compared to the healthy ones. ( B ): PTEN was upregulated in the tumorous samples compared to the healthy ones in the first and third samples, while in sample two and four PTEN expression was downregulated.

Article Snippet: Blotting membranes were blocked with 5% milk-TBS-Tween for 1 h at room temperature and then incubated overnight with the specific primary antibodies at 4 °C (IDO, D5J4E in 1:1000 dilution, BOSTER Biological Technology, Pleasanton, CA, USA; PTEN (D4.3) XP(R) Rabbit mAB, in 1: 1000 dilution, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Isolation, Electrophoresis, SDS Page, Software